Part:BBa_K277128
3L.3_23.D3.07
3L.3_23.D3.07 is 714 bases long and is cloned into the pGem-T vector.
3L.3_23.D3.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.D3.07 is a constituent of 3L.3_23.D3 (along with 3L.3_23.D3.01, 3L.3_23.D3.02, 3L.3_23.D3.03, 3L.3_23.D3.04, 3L.3_23.D3.05, 3L.3_23.D3.06, 3L.3_23.D3.08, 3L.3_23.D3.09, 3L.3_23.D3.10, 3L.3_23.D3.11, and 3L.3_23.D3.12.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
gene YCL001W (-208..358) Protein involved in retention of membrane proteins%2C including Sec12p%2C in the ER%3B localized to Golgi%3B functions as a retrieval receptor in returning membrane proteins to the ER
loxP_site loxPsym_YCL001W (362..395)
reverse_primer YCL001W_tagr1v1 (169..196)
Sequence (corresponds to coordinates 92971..93684 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 110
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 110
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 110
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 110
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 110
- 1000COMPATIBLE WITH RFC[1000]
| None |